Over the past years, the possibility to extend the concept of magnetic guidance to tissue engineering has opened an exciting wide research area of interest. The aim of the present work was to assess morphological, mechanical, magnetic and biological performances of nanocomposite magnetic scaffolds obtained through 3D fiber deposition and stereolithography techniques. These techniques may provide a morphologically-controlled and tailored structure. A superparamagnetic scaffold should provide the possibility to magnetically "switch-on/switch-off" it in order to deliver biofactors/stem cells, or to stimulate cell adhesion, proliferation and differentiation. 3D nanocomposite magnetic scaffolds were suitably prepared. The performances of the designed scaffolds were assessed by means of experimental/theoretical investigations. In particular, morphological studies were performed through micro-computed tomography and scanning electron microscopy and mechanical analyses through tensile and compression tests. Human mesenchymal stem cells were seeded on 3D scaffolds in order to study the effect of a static and a time-dependent magnetic stimulation on cell adhesion/spreading. Morphological analyses highlighted that these techniques are able to manufacture well defined scaffolds. Results from mechanical analyses showed that beyond a specific limit of MNP amount, the mechanical performances of the scaffolds decrease. Biological analyses showed that prolonged exposure time to the static or dynamic magnetic field seems to negatively affect cell viability. Confocal analyses highlighted interesting results in terms of cell adhesion/spreading. Alamar Blue assay and Alkaline-phosphatase activity provided a quantitative evaluation of cell viability/proliferation and differentiation, respectively over the culture time.