A large variety of processes and tools is continuously investigated to discover new solutions to design instructive materials with controlled chemical, physical, and biological properties for tissue engineering and drug delivery. Among them, electrohydrodynamic techniques (EDT)are emerging as an interesting strategy, based on highly flexible and low-cost processes, to revisit old biomaterial’s manufacturing approach by utilizing electrostatic forces as the driving force for thefabrication of 3D architectures with controlled physical and chemical functionalities to guide in vitro and in vivo cell activities.By arational selection of polymer solution properties and processconditions, EDTs allow to produce fibres and/or particles at micro and/or nanometric size scale which may be variously assembled by tailored experimental setups, thus giving the chance to generate a plethora of different 3D devices able to incorporate biopolymers (i.e, proteins, polysaccharides) or molecules (e.g., drugs, growth factors) for different applications [1].Here, we focus on the optimization ofbasic EDTs - namely electrospinning, electrospraying and electrodynamic atomization -to develop active platforms(i.e, monocomponent, protein anddrug loaded scaffolds and µ-scaffolds) made of synthetic (i.e, PCL, PLGA) or natural (i.e, chitosan, alginate) polymers. In particular, we investigate how to set materials and process parameters to impart specific morphological, biochemical or physical cues to trigger all the fundamental cell–biomaterialand cell–cell cross-talking elicited during regenerative processes, in order to reproduce the complex microenvironment of native or pathological tissues. Acknowledgements: POLIFARMA (PON02_3203241)and NEWTON (FIRB-RBAP11BYNP), REPAIR (PON01-02342). References: [1] Guarino V. et al - Expert Rev Med Devices. 2015 Jan; 12(1):113-29.